It’s funny b/c Spider-Man making no quips is usually code for “someone fucked up and Spidey’s on the warpath”. That’s probably why the fourth guy just said “uh-oh”.

Spider-Man’s humor is so prevalent that when Superior Spider-Man wrapped the Green Goblin knew that it was Parker back in his own head simply because Spidey joked with him. 

This is why I love Spider-Man